Nonlinear microscopy is a powerful technique for imaging inside living organisms with submicrometer resolution at millimeter depths. Combined with genetically encoded calcium indicators and opsins, multiphoton microscopy has revolutionized neuroimaging and is becoming a standard tool in neuroscience.
Image above: CRONUS-2P femtosecond laser with Thorlabs Bergamo microscope.
Join experts from Light Conversion and Thorlabs for a webinar hosted by Rosh Electroptics. Together, we will explore how multiphoton microscopy and ultrafast lasers are driving breakthroughs in neuroscience – enabling deeper imaging, higher resolution, and more advanced experimental workflows.
Tuesday, 21 October 2025
12:00 (EET) | 09:00 (GMT)
Designed for researchers, imaging specialists, and lab managers, this webinar brings together two global leaders in ultrafast technology to present practical insights, proven approaches, and new solutions for neuroscience research.
SPEAKERS & TOPICS
Femtosecond Lasers for Advanced Multiphoton Imaging
Mantvydas Mikulis | Product Manager, Light Conversion
- The role of CRONUS and CARBIDE femtosecond lasers in two- and three-photon imaging
- Key laser parameters that influence imaging quality
- Practical optimization strategies to save valuable research time
- Case examples from collaborations with researchers and microscopy companies
Advanced Applications of Multiphoton Microscopy in Neuroscience
Dr. Sergey Matveev | Thorlabs
- Deep tissue imaging strategies beyond conventional two-photon limits, including three-photon techniques
- Customization of table optics and objective selection for different imaging needs
- Integration of imaging with optogenetics, holographic stimulation, and synchronized workflows.
HOST
Dr. Eldad Ben-Ishay | Imaging and Microscopy Specialist, Rosh Electroptics
Image: Comparison of in vivo 2P and 3P calcium imaging of mouse visual cortex GCaMP neurons on a Thorlabs Bergamo II microscope using a typical 2P laser and CRONUS-3P laser at 920 nm and 1300 nm, respectively.
Courtesy of CSHL ISFNS 2024 school organizers and Willis Broden Jr. and Sergey Matveev (Thorlabs).