Lastek: Photonics Technology Solutions

In the current Issue of Imaging & Microscopy (p.46, Volume 19, March 2017) Abberior present their Compact Line STEDYCON (shown above). Article available online here: "STED in a Shoebox. Ultra-Compact Microscopes with Unprecedented User-Friendliness“. 

In recent years, fluorescence microscopy has undergone a revolution: the resolution barrier given by the diffraction limit was broken by new microscopy concepts such as STED, GSD, GSDIM, PALM, STORM and RESOLFT. Abberior Instruments, co-founded by 2014 Nobel Prize in Chemistry winner, Professor Stefan W. Hell,  has rapidly become the leading supplier of cutting-edge super-resolution technology with the best possible resolution performance. In combination with their sister company Abberior, they offer an unrivaled combination of superresolution dyes and nanoscopy hardware. 

Based on more than two decades of research experience in superresolution microscopy / fluorescence nanoscopy and a passion for developing novel concepts and methods in superresolution imaging for this new era of microscopy, the Abberior Instruments team is able to offer novel superresolution microscopes for general applicability in the live sciences. In Australia and New Zealand, Abberior has partnered with Lastek to offer the most complete experience for super resolution microscopy.  Contact us to discuss your application today. Read more on systems from Abberior Instruments and other accessories for super resolution microscopy at Lastek below.

• STED 
• FLIM 
• RESOLFT 
• Correlative STED + AFM
• Fluorophores for Super Resolution Microscopy 
• Nanobeads and Nanorulers  

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Abberior Instruments is proud to announce its new easy3D STED module which uses a completely novel approach to 3D STED microscopy. At its core, a programmable spatial light modulator (SLM) is used to create the phase patterns required for 2D and 3D STED microscopy. At the same time, it can also be used to correct for optical aberrations.

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Two-color superresolution STED microscope with a pulsed STED laser @ 775 nm

• Multicolor confocal scanning system with variable pinhole size
• Detector gating with up to 4 gates
• Accessible optomechanical design, open electronics and software platform, ready to implement your own imaging ideas
• Zero-compromize on performance - get the best resolution performance possible in STED microscopy
• QUAD beam scanner technology
• We offer unrivaled flexibility in customizing the microscope to your applications

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Two-color superresolution STED microscope with a pulsed STED laser @ 595 nm

• Multicolor confocal scanning system with variable pinhole size
• Detector gating with up to 4 gates
• Accessible optomechanical design, open electronics and software platform, ready to implement your own imaging ideas
• Zero-compromize on performance - get the best resolution performance possible in STED microscopy
• QUAD beam scanner technology
• We offer unrivaled flexibility in customizing the microscope to your applications

We install Your turn-key Abberior 2-color STED 595 QUAD scanning nanoscope in your lab including software/handling training.

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Sample: keratin19 (rsCherryRev1.4, red) and vimentin (Dronpa-M159T, green)
Resolution RESOLFT < 80 nm

• Two superresolution parallel RESOLFT channels: one ‘green channel’ using e.g. rsEGFP or rsEGFP2 and one ‘red channel’ using e.g. rsCherryRev
• Multicolor widefield system using two sCMOS detectors
• Accessible optomechanical design, open electronics and software platform, ready to implement your own imaging ideas
• Zero-compromize on performance - get the best resolution performance possible in RESOLFT microscopy
• Ultraparallel RESOLFT version using >100.000 donuts
• We offer unrivaled flexibility in customizing the microscope to your applications

We install your turn-key 2-color parallel RESOLFT nanoscope in your lab including software/handling training.

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Abberior Instruments Superresolution STED microscope with a pulsed STED laser @ 775 nm

• Confocal scanning system
• QUAD beam scanner technology
• Detector gating with up to 4 gates
• Accessible optomechanical design, open electronics and software platform, ready to implement your own imaging ideas
• Zero compromise on performance - get the best resolution performance possible in STED microscopy

We offer unrivaled flexibility in customizing the microscope to your applications

We install your turn-key Abberior STED 775 QUAD scanning nanoscope in your lab including software/handling training.

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Sample: keratin19 (rsCherryRev1.4, red) and vimentin (Dronpa-M159T, green)
Resolution RESOLFT < 80 nm  

One-color superresolution green RESOLFT point scanning system for switchable GFPs

• Multicolor confocal system with variable pinhole size
• Accessible optomechanical design, open electronics and software platform, ready to implement your own imaging ideas
• Zero-compromize on performance - get the best resolution performance possible in RESOLFT microscopy
• QUAD beam scanner technology
• We also offer unrivaled flexibility in customizing the microscope to your applications.

We install your turn-key RESOLFT scanning nanoscope in your lab including software/handling training.

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Quad Scanner design with 4 galvo mirrors

Features:

• Patent protected scanning technology
• Novel four-mirror design
• Compact and robust scan head
• No scan lens required:
• minimal wavefront distortions
• Beam alignment by software
• 4 degrees of freedom: beam
• position & angle independently addressable 
• Scan field: > 80 x 80 ?m
• Line frequency: up to 2 kHz

Controlling platform for Abberior Instruments microscopes

Abberior software platform Inspector

Microscope control:

• RESOLFT and STED image acquisition
• QUAD scanner control
• Laser timing and data collection for pulse-, pixel-, and line-interleaved multiplexing
• Control of all motorized components (pinhole, mirrors, flipper, shutters, microscope body
• Ready to control your own lasers and optomechanics

Image analysis:

• Analysis toolbox for quantitative microscopy
• Linear and non-linear (RL) deconvolution algorithms
• Abberior Patchpanel for synchronized Multi-IO
• 72 configurable signal connectors
• 12 connectors for ultrafast signals for laser pulsing and gated detection
• Typical Abberior configuration leaves 40+ connectors for your custom application

Abberior Instruments RESCue STED is a STED imaging mode dedicated to live-cell imaging with saving up to 96% of the light dose without compromising the resolution.  

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