Light Conversion Microscopy Sources

Light Conversion CRONUS-2P: Three-Channel Wavelength-Tunable Femtosecond Laser

Light Conversion's CRONUS-2P is a three-channel tunable femtosecond laser providing watt-level three synchronized outputs with high repetition rate, short pulse duration, and GDD control, making it the ultimate source for nonlinear microscopy. In standard configuration, the two independently tunable outputs, operating separately or simultaneously, cover 680 – 960 nm and 940 – 1300 nm, respectively, while the third is fixed at 1025 nm and is accessible in parallel. Thus, the CRONUS-2P can be used for simultaneous excitation of multiple fluorescent probes, calcium indicators, or opsins at their absorption maxima, whereas second- and third‑harmonic emission (SHG and THG) can be spectrally shifted for ease of detection or resonant enhancement.

Light Conversion CRONUS-3P: Laser Source for Advanced Nonlinear Microscopy

Light Conversion's CRONUS-3P is a laser source developed for advanced nonlinear microscopy. It provides µJ-level pulses down to 50 fs at repetition rates of up to 2 MHz and tunable in the short-wavelength infrared (SWIR) range from 1250 to 1800 nm, thus covering the biological transparency windows at 1300 and 1700 nm for three-photon (3P) microscopy. In addition, CRONUS-3P offers integrated GDD control and beam steering, as well as simultaneous 1030nm nm output.

Typically, multiphoton imaging in the SWIR range requires a complex multi-device laser system, a large optical table, and skilled staff. This reality encumbers neuroscience and other biomedical applications. The CRONUS-3P system is a next-generation, industrial-grade, single-supplier solution that is more compact, more reliable, and more versatile. It achieves tunable femtosecond excitation with integrated group delay dispersion (GDD) control, ensuring optimal pulse duration at the sample, while industrial-grade design guarantees high pulse-to-pulse energy and long-term power stability.

Light Conversion Microscopy Sources: Cronus-2P and Cronus-3P Tunable Femtosecond Laser Systems

Light Conversion’s product portfolio features microscopy-dedicated femtosecond laser sources CRONUS-2P and CRONUS-3P.

These lasers cover applications in functional neuroimaging, optogenetics, and deep imaging using medium-repetition-rate three-photon excitation and fast high‑repetition-rate two-photon imaging, as well as widefield and holographic excitation using high-power laser sources.  

Light Conversion: New CRONUS-2P Models to Advance Multiphoton Microscopy

Nonlinear microscopy is a powerful technique for imaging inside living organisms with submicrometer resolution at up to millimeter depths. In combination with genetically encoded fluorescent indicators and opsins, multiphoton microscopy has revolutionized intravital and neuroimaging and is becoming a standard tool in bioscience.

To further increase support for these demanding applications, Light Conversion is expanding its CRONUS microscopy-dedicated line of femtosecond lasers with two additional CRONUS-2P configurations. The new models offer greater flexibility in selecting excitation sources optimized for diverse experimental requirements – allowing users to focus on their research rather than the light source behind it.