In two-photon microscopy, simultaneous multi-colour imaging provides rich insight into biological samples . Especially, metabolic imaging greatly benefits from simultaneous NADH and FAD measurements. With the Toptica FemtoFiber ultra dual-colour, two FemtoFiber ultra are optically synchronized and allow simultaneous imaging with two different laser colours. No laser wavelength tuning is required. A shared oscillator design guarantees all-optical synchronization and hence a fixed, defined delay between the two laser colours with minimum jitter. In addition, only a single electronic trigger output serves as reference for TCSPC electronics in Fluorescence lifetime imaging (FLIM) as well as gated detectors.
- Two synchronized laser lines for multi-colour non-linear imaging
- Shared oscillator design for all-optical synchronization
- Fixed delay between two colours with minimum jitter
- Single electronic trigger output as reference for TCSPC and gated detection
Applications:
- Two-Photon Fluorescence Microscopy
- SHG microscopy
- FLIM
- Spectral unmixing
- Neuroscience
- Pump and probe
Specifications:
| Center wavelength | 920 nm and 780 nm | 920 nm and 1050 nm |
| Average output power | > 1 W at 780 nm, > 1.5 W at 920 nm |
> 1.5 W at 920 nm, > 5 W at 1050 nm |
| Pulse duration | <150 fs W at 780 nm, < 100 fs at 920 nm |
< 100 fs at 920 nm, < 100 fs at 1050 nm |
| Repetition rate | 80 MHz | |
| Motorized dispersion precompensation (GDD) | -30,000 fs² ... 0 fs² at 780 nm, -40,000 fs² ... +1,000 fs² at 920 nm, independent per wavelength |
-40,000 fs² ... +1,000 fs², independent per wavelength |
| Integrated power control (AOM, optional) | > 1 MHz AOM modulation bandwidth, independent per wavelength, analogue and digital modulation available | |
| Dimensions supply unit | Twice 131 x 484 x 600 mm³ (19 inch rack format, 3U) | |
| Dimensions laser head | Twice 77 x 165 x 300 mm³ (H x W x D) | |