The next generation of electrophoretic tissue-processing devices, SmartBatch+ from LifeCanvas Technologies and new buffers have improved the removal of lipid membranes to render a sample optically transparent for lightsheet imaging.
Tissue clearing techniques have long since sought to maximize optical transparency while preserving sample architecture and minimizing processing time. While other techniques are forced to balance these objectives, LifeCanvas Technologies have developed a technique that makes no such compromises. Delipidation and optical clearing without expansion or shrinking of the samples throughout the entire process with targeted removal of lipid membranes and minimal impact on other structures has been achieved.
The sample retains its mechanical strength, molecular stability, and native 3-dimensional structure. This is the only method available that can clear whole mouse brains and larger samples without any change in morphology, to our knowledge.
This targeted approach also features a drastic increase in delipidation speed. We can now fully delipidate whole mouse brains in just one week with a passive incubation at 45°C, or with one day of active clearing in SmartBatch+ or SmartClear II Pro at 42°C. This increase in speed also extends to whole rat brains, achieving active delipidation in ~4 days at 42°C.
SmartBatch+ actively clears and labels up to 12 whole mouse brains or comparably sized samples or 2 whole rat brains simultaneously leading to consistent histological data!
SmartBatch+ is also very cost effective by using just 4-20 μg of antibody per target to label a sample the size of a whole mouse brain — up to an order of magnitude less than passive staining.
These are significant savings per sample per antibody!
Electrophoretic tissue clearing & immunolabeling
SmartBatch+ combines active tissue clearing and immunolabeling into one high-throughput device! Our easy-to-use system allows you to switch between clearing and labeling modes simply by changing buffers. SmartBatch+ is compatible with different tissue types and large intact samples.
An advance on our founder’s CLARITY tissue clearing method, our Clear+ technique provides maximum optical transparency with no tissue expansion or contraction. In immunolabeling mode, eFLASH and patented SE technologies uniformly immunolabel whole organs. Unlike organic methods (e.g. iDISCO) or other aqueous methods (e.g. CUBIC), SmartBatch+ actively preserves fluorescent protein signal.
Consistent histological data
SmartBatch+ actively clears and labels up to 12 whole mouse brains or comparably sized samples in as little as one day, and 2 whole rat brains in just 3-4 days! This provides unparalleled consistency for multiple treatment groups. Its small footprint also allows ample space for multiple devices.
High quality sample integrity for 3D imaging
SmartBatch+ combined with SHIELD tissue preservation protects fluorescent protein signal, antigenicity, and molecular and physical architecture through all tissue processing steps. Clear+ is also currently the only tissue clearing technique that preserves sample morphology. This ensures your samples will produce information-rich images.
Fast, cost-effective clearing & immunolabeling
SmartBatch+ uses just 4-20 μg of antibody per target to label a sample the size of a whole mouse brain — up to an order of magnitude less than passive staining. That’s hundreds of dollars in savings per sample per antibody, in primary antibody alone! Its turnkey design is also labor-efficient, requiring <12 mins hands-on time per sample to clear and label.