Fluorescent beads are important microscopy tools which can be used for calibration of microscopes, particle tracking or quantitative determination of point spread functions especially in STED microscopy. Particularly for the last point a small structure size keeping a high brightness is an immense advantage. Products from the Gattaquant GATTA-Bead series fulfill this requirement perfectly with a diameter of only 23 nm. Additionally, they show a clearly improved homogeneity and flexibility compared to other beads. We offer GATTA-Beads in the colors red (ATTO 647N), orange (ATTO 594), green (ATTO 542) and blue (Oregon Green 488). Moreover, we offer alternative dyes which are indicated on the table below.
On request we can also design special solutions for your specific requirements. All samples will be delivered on a sealed glass slide which you can comfortably put directly on your microscope.
Fields of Application:
- Setup calibration
- 2D STED measurements
- 3D STED PSF distillation and deconvolution
- Tracking and drift-correction
Advantages:
- Size: d = 23 nm
- Brightness: the highest brightness density
- Homogeneity: super small size and intensity distribution
- Flexibility: change color or add chemical groups
Fluorophore Properties:
| Fluorophore | Our notation | Excitation / nm | Emission / nm |
| ATTO 647N | R | 647 | 664 |
| Abberior Star 635P | R | 635 | 651 |
| ATTO 594 | O | 594 | 626 |
| ATTO 542 | G | 542 | 562 |
| Cy3 | G | 554 | 568 |
| Alexa Fluor 555 | G | 555 | 580 |
| Oregon Green 488 | B | 488 | 526 |
| Alexa Fluor 488 | B | 488 | 525 |
| ATTO 490LS | B | 490 | 658 |