The Life Canvas Technologies MegaSPIM microscope is their newest light sheet imaging system. Utilizing the same powerful axial sweeping technology as SmartSPIM, MegaSPIM is optimized for imaging not only laterally large samples, but thin slices and arrays of small tissues as well.
Unique highlights and features include:
- Capacity for lateral sample sizes up to 200 mm x 200 mm – easily image expanded tissues, large slabs, and arrays of organoids, slices, or whole rodent organs
- Custom mounting solutions for a variety of sample types
- High-throughput multicolor acquisition with dual cameras and independent focus correction
- Post-processing tools to deskew and stitch your data into complete ready-to-analyze volumes
MegaSPIM is a highly flexible imaging system designed to rapidly acquire high-resolution data from a variety of tissue types, including: thin sections, cleared thick sections of human or nonhuman primate organs, and arrays of smaller cleared samples such as whole rodent organs or organoids. We offer custom mounting solutions for all of these tissues and more.
After image acquisition, our streamlined post-processing pipeline facilitates destriping, deskewing, and stitching large data sets. Easily create complete 3D image sets that are ready for analysis.
High-throughput volumetric imaging, designed for speed and versatility
Easily scan through your tissues and transform raw images into complete 3D stacks ready for viewing and analysis. With optional dual camera acquisition, efficiently generating high-quality data from multiple or larger samples has never been easier.
3-channel acquisition of large samples, like the 2mm-thick pig brain section below, can be completed in just 4 hours with the 1.8X objective and a single camera.
Light sheet illumination and detection
MegaSPIM uses patented axial sweeping technology to capture images with uniform axial resolution across the entire field of view without deconvolution. Long working-distance objectives positioned at 45 degrees above the sample plane allow imaging of samples as laterally large as the travel range of the stage.
Our deskewing algorithm seamlessly translates these tilted image stacks into standard XY planes, ready to be stitched into complete XYZ stacks for visualization and analysis.
Download datasheet here.
More than a microscope – a complete solution
Tissue Processing: Prepare samples with tools for preservation, clearing, labeling, & sectioning.
Light Sheet Imaging: Acquire spatially informative images with innovative microscopy techniques.
Image Analysis: Generate publication-quality data with robust analysis tools.
