Fluorescence lifetime imaging microscopy or FLIM is an imaging instrument type for producing an image based on the differences in the exponential decay rate of the fluorescence from a fluorescent sample. Fluorescence lifetime imaging yields images with the intensity of each pixel determined by τ, which allows researchers to view contrast between materials with different fluorescence decay rates, and also produces images which show changes in other decay pathways.

Fluorescence lifetimes can be determined in the time domain by using a pulsed source. When a population of fluorophores is excited by an ultrashort or delta pulse of light, the time-resolved fluorescence will decay exponentially. Time-correlated single-photon counting (TCSPC) is usually employed as a measurement method because it compensates for variations in source intensity and single photon pulse amplitudes. More specifically, TCSPC records times at which individual photons are usually detected by a single photon avalanche photo diode (SPAD) with respect to the excitation laser pulse.

The recordings are repeated for multiple laser pulses and after enough recorded events, researchers are able to build a histogram of the number of events across all of these recorded time points. This histogram can then be fit to an exponential function that contains the exponential lifetime decay function of interest, and the lifetime parameter can accordingly be extracted. Nanobase's Xper-FLIM is equipped with the single-photon avalanche diode (SPAD)- TCSPC FLIM system and can offer options of detection channels at lowcost, realizing super fast analysis for FLIM researchers. Xper-FLIM is also available in either an inverted microscope or an upright microscope to meet a recently growing number of various application needs.

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Features

  • Confocal/3D fluorescence lifetime imaging and analysis
  • Fast 2D scanning and bright field microscopic imaging
  • Various options of VIS laser/filter
  • Time-correlated single photon counting
  • Time-resolved photoluminescence (TRPL) measurement

Components

  • ​Scanner module
  • Microscope
  • Picosecond pulsed diode laser and driver
  • Main body
  • SPAD-TCSPC system
  • NanoSpectrum analysis software

Applications

  • ​Biology
  • Medical research
  • Semiconductors
  • Photoelectron materials
  • Solar cells