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Abberior Instruments RESCue STED enables superresolution imaging of the malaria infection cycle

 figure rescue sted komplett520

Samples courtesy of Dr. Michal Pasternak, WEHI Melbourne, Australia

figure final.1280x0

Malaria-infected erythrocytes containing parasite proteins (Abberior STAR635P immunostaining, magenta) and hemozoin particles (yellow). RESCue STED reveals structures smaller than 39nm while avoiding laser induced sample damage. Samples prepared by Dr. Michal Pasternak, WEHI Melbourne, Australia.

Live-cell STED with REduction of State transition Cycles (RESCue STED)

Intelligent light dose management

  • RESCue avoids unnecessary excitation and de-excitation cycles and thus reduces photobleaching of any fluorescent marker
  • The reduction in excitation and de-excitation cycles is particularly beneficial for volume imaging with 3D STED as well as for time lapse STED imaging
  • RESCue STED applies excitation and STED light only at positions where fluorescent markers (i.e. the target structure) is present. It shuts off the lasers at positions where exposure would not make sense (i.e. where no markers are found)
  • The resolution of the image is maintained although the light dose is reduced down to 4 % compared to gated cw STED imaging and down to 20 % compared to pulsed STED imaging
  • RESCue STED is a highly photon-efficient way of superresolution imaging while conventional STED applies the same light dose to any pixel no matter if markers (i.e. a target) are present or not
  • RESCue can be applied with similar benefits also to confocal imaging

For further information, please see here

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