Abberior Instruments 1-color STED 775 QUAD Scanning
Abberior Instruments Superresolution STED microscope with a pulsed STED laser @ 775 nm
- Confocal scanning system
- QUAD beam scanner technology
- Detector gating with up to 4 gates
- Accessible optomechanical design, open electronics and software platform, ready to implement your own imaging ideas
- Zero compromise on performance - get the best resolution performance possible in STED microscopy
We offer unrivaled flexibility in customizing the microscope to your applications
We install your turn-key Abberior STED 775 QUAD scanning nanoscope in your lab including software/handling training.
Sample: nuclear pore complex protein (gp210)
Resolution STED < 25 nm
Abberior specifications:
- Pulsed STED laser (775 nm) featuring one super-resolution STED channel
- Resolution up to 20 nm; Typical resolution < 30 nm
- Individual upgrade options, e.g. multicolor, 3D-STED, additional STED laser,... and future innovations
- Manual Olympus IX81 microscope body (or other stand on request)
- Piezo stage for z scanning
- Pulsed excitation laser (640 nm)
- 1-color LED widefield illumination
- choose between a monochrome widefield camera or an ocular
- Abberior QUAD scanner: novel scanner design with 4 galvo mirrors provides 4 degrees of freedom for independent position and angle settings; line frequency up to 2 kHz
- Abberior Patch Panel for interfacing additional custom devices (e.g. lasers etc.)
- Suitable for superresolution imaging with organic dyes such as Abberior STAR 635P or ATTO 647N
- Up to 8 time gating channels with <80 ps time resolution
- Up to 4 pulse-timing channels with <80 ps time resolution
- Imspector image acquisition and data analysis software (includes smoothing, deconvolution, etc.)
Download pdf Brochures:
Abberior Instruments Product Brochure
References
- Kolmakov, K., C. A. Wurm, D. N. H. Meineke, F. Göttfert, V. P. Boyarskiy, V. N. Belov, S. W. Hell: "Polar Red-Emitting Rhodamine Dyes with Reactive Groups: Synthesis, Photophysical Properties, and Two-Color STED Nanoscopy Applications" Chem. Eur. J. 20, 146 - 157 (2014)
- Clausen, M.P., Galiani, S., de la Serna, J.P., Fritzsche, M., Chojnacki, J., Gehmlich, K., Lagerholm, B.C., Eggeling, C.:"Pathways to optical STED microscopy", DOI: 10.2478/nbi-2013-0001, NanoBioImaging, 1-12 (2013)
- Göttfert, F., Wurm, C.A., Mueller, V., Berning, S., Cordes, V.c., Honigmann, A., and Hell, S.W.: "Coaligned Dual-Channel STED Nanoscopy and Molecular Diffusion Analysis at 20 nm Resolution", Biophysical Journal Volume 105, L01–L03 (2013)
- Berning, S., K. I. Willig, H. Steffens, P. Dibaj, S. W. Hell: "Nanoscopy in a Living Mouse Brain" Science 335, 551 (2012)
- Wurm, C. A., K. Kolmakov, F. Göttfert, H. Ta, M. Bossi, H. Schill, S. Berning, S. Jakobs, G. Donnert, V. N. Belov, S. W. Hell: "Novel red fluorophores with superior performance in STED microscopy" Optical Nanoscopy 1, 1 – 7 (2012)
- Wildanger, D., B. R. Patton, H. Schill, L. Marseglia, J. P. Hadden, S. Knauer, A. Schönle, J. G. Rarity, J. L. O’Brien, S. W. Hell, J. M. Smith: "Solid Immersion Facilitates Fluorescence Microscopy with Nanometer Resolution and Sub-Ångström Emitter Localization" Adv. Mater. 24, 309 - 313 (2012)
- Kolmakov, K., C. A. Wurm, R. Hennig, E. Rapp, S. Jakobs, V. N. Belov, S. W. Hell: "Red-Emitting Rhodamines with Hydroxylated, Sulfonated, and Phosphorylated Dye Residues and Their Use in Fluorescence Nanoscopy" Chemistry", A European Journal 18, 12986 - 12998 (2012)
- Reuss, M., J. Engelhardt, S. W. Hell (2010): "Birefringent device converts a standard scanning microscope into a STED microscope that also maps molecular orientation". Opt. Exp. 18 (2), 1049-1058 (2010)
- Harke, B., C. Ullal, J. Keller, S. W. Hell: "Three-Dimensional Nanoscopy of Colloidal Crystals" Nano Lett. 8, 1309-1313 (2008)
- Harke, B., J. Keller, C. K. Ullal, V. Westphal, A. Schönle, S. W. Hell: "Resolution scaling in STED microscopy", Opt. Express16, 4154-4162 (2008)
