3D-protected STED imaging of neuron in living brain tissue. 3D-rendering of a 3D-image stack of a living neuron expressing the AC-rsEGFP2 actin label in a hippocampal brain slice. Protected STED was applied with resolution enhancement in the xy and z directions. The image shows a stretch of dendrite with dendritic spines. The stack corresponds to 56 z slices spaced by 80 nm. Scale bar: 1 µm.
Abberior Instruments Protected STED technique has been highlighted in a new January 2016 paper in Nature Photonics. Full paper available for download here.
Coordinate-targeted Fluorescence nanoscopy with multiple off states
Johann G. Danzl*†, Sven C. Sidenstein†, Carola Gregor, Nicolai T. Urban, Peter Ilgen, Stefan Jakobs and Stefan W. Hell*
Nature Photonics: Published Online 18 January 2016 DOI: 10.1038/NPHOTON 2015.266
Abstract: By exploiting a second off state of a reversibly switchable fluorophore, a general approach that can reduce photobleaching and enhance resolution of coordinate-targeted fluorescence nanoscopy has been demonstrated.
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