Lastek: Photonics Technology Solutions

CoolLED’s pE-300white offers intense, broad-spectrum LED illumination for imaging most common fluorescent stains.  The pE-300whiteLight Source can be fitted directly to the microscope or specified with a liquid light guide.  The system is a mercury-free alternative that is safer, more controllable, and repeatable than conventional high-pressure gas discharge illuminators.  Spectral coverage is from the UV (DAPI excitation) to the Red region (Cy5 excitation). The system is perfect for hospital and regular research laboratory environments.

The pE-300white is a compact and simple to use LED illumination unit.  It provides a broad spectrum of illumination,  covering the excitation bands of common fluorescent stains.  Operation is by a remote manual control pod with instant on/off and intensity control from 0-100%.  Remote control is available via USB or TTL trigger.

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The CoolLED pE-100 series is a mercury-free family of compact and simple to use LED illumination systems which can be configured to deliver light directly to a microscope, or via a liquid light guide or multimode fiber. Systems can be specified at any one of 20 different LED wavelengths.

Operation is by a remote manual control pod with instant on/off and intensity control from 0-100%. Remote control is available via a TTL trigger.

The system has been developed for applications requiring a single LED excitation wavelength.  It is perfect for clinical applications such as routine screening (e.g. using Auramine for Tuberculosis), for research applications requiring precise intensity control and fast-switching, or for electrophysiology applications where light has to be delivered to a specific location.  The user can select from 20 different LED wavelengths, ranging from the near-UV at 365nm to the near-IR at 770nm.  The system comprises a pE-100 LED Light Source, control pod, and power supply.

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The CoolLED pE-4000 sets the standard as the universal illumination system for fluorescence microscopy.  The system has 16 selectable LED wavelengths that can be matched to the filters and fluorophores of almost any microscope, making it the broadest spectrum of illumination available. For the user who wants consistency and ease of use with simple on/off and intensity buttons, the pE-4000 can be operated as a ‘White’ illumination system. For the user who wants total control over the excitation source to improve imaging, individual wavelengths settings can be selected and controlled in “Advanced” mode.

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Mad City Labs recently introduced two new, low cost microscopy solutions. The MCL-MOTNZ motorized XY stage with piezo Z and MCL-MANNZ manual XY stage with piezo Z are compatible with major third party microscopy and instrumentation control software including LabVIEW, Nikon Elements, Metamorph, ?Manager, SlideBook, and Image-Pro. 

The MCL-MOTNZ is a value priced positioning system for use with inverted optical microscopes. The MCL-MOTNZ combines a stepper motor XY linear motion stage with a closed loop, high resolution, Z-axis nanopositioning system. The micropositioning stage provides 25 mm of travel per axis with a minimum step size of 95 nm. The use of high precision components and our proprietary intelligent control technique results in an ultra-stable microscope platform with excellent native precision without the addition of costly encoders. The innovative sample holder design together with the ultra-low profile of the piezo Z stage makes sample exchange and sample loading quick and straightforward.

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• Easy installation
• Very economical
• High resolution live cell imaging

LCI (Live Cell Instrument, South Korea) offer a range of innovative observation chambers and incubator systems under the Chamlide brand, and are now available in Australia exclusively from Lastek. 

Chamlide Magnetic Chambers use a patented magnetic system that allow for quick and easy assembly. They are semi-permanent, replacing consumable culture-ware and expensive coverslip-bottomed dishes.

Chamlide magnetic chambers use coverslips (thickness 0.15-0.20 mm), allowing for high resolution imaging.

Why observation chamber with coverslip?

According to Abbe's theory, the resolution of light microscope fully depends on the N.A. of the objective lens. Therefore, for high resolution microscopy, it is absolutely required to use high N.A. objective lenses, which unavoidably have very short working distance. The conventional plastic culture wares are not thin enough to use with a high N.A. lens; thus, one should use a coverslip with a thickness in the range of 0.15-0.20 mm. Besides, a coverslip does not emit auto-fluorescence and has an appropriate reflective index (~1.515) for use with oil or water immersion objective lenses.

Scientific Camera integrated into 60mm Cage System

Thorlabs' new 4 Megapixel CCD Cameras, which offer up to 13 frames per second at 40 MHz dual-tap readout of the full sensor, are specifically designed for microscopy and other demanding scientific imaging applications. These monochrome cameras are ideal for multispectral imaging, fluorescence microscopy, and other high-performance imaging techniques that would benefit from a large field of view, high quantum efficiency, and low noise sensor.

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The accuracy and speed of probe positioning is a critical and important value for microscopy techniques such as laser scanning microscopy, or other techniques including fluorescent, super resolution, or image processing. In order to provide the user with a tool able to improve these techniques, piezosystem jena provides the new PZ 300 piezoelectric actuator based z-axis elevator stage for microscopes.

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Mad CIty Labs' RM21™ is a precision aligned microscope platform for epifluorescence microscopy. The RM21™ has been designed for maximum user accessibility. This feature offers users the opportunity to develop flexible configuration microscopy instruments with ease. It has been manufactured with high precision to allow easy alignment of microscopy and optical components within its three dimensional space. In addition, all posts and fixturing points are referenced to a known datum. With a robust design, precision manufacturing and assembly, the RM21™ is the ideal platform for a range of microscopy applications such as super resolution (SR) microscopy, fluorescence microscopy and TIRF.

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The Mad City Labs SPM-M Kit combines the MadPLL® instrument package with Mad City Labs high resolution nanopositioning systems to form a high performance, closed loop, scanning resonant probe microscope. The seamless integration of hardware combined with the built-in automated control of MadPLL® means that you can concentrate on getting results, not tweaking parameters. Applications for the SPM-M Kit include nanoscale characterization and nanoscale fabrication applications such as optical antennas, nano-optics, semiconductors, data storage, and more. The SPM-M Kit is ideal for research and teaching laboratories offering high performance, versatility, simplicity and excellent value.

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CoolLED’s pE-300white offers intense, broad-spectrum LED illumination for imaging most common fluorescent stains.  The pE-300whiteLight Source can be fitted directly to the microscope or specified with a liquid light guide.  The system is a mercury-free alternative that is safer, more controllable, and repeatable than conventional high-pressure gas discharge illuminators.  Spectral coverage is from the UV (DAPI excitation) to the Red region (Cy5 excitation). The system is perfect for hospital and regular research laboratory environments.

The pE-300white is a compact and simple to use LED illumination unit.  It provides a broad spectrum of illumination,  covering the excitation bands of common fluorescent stains.  Operation is by a remote manual control pod with instant on/off and intensity control from 0-100%.  Remote control is available via USB or TTL trigger.

Read more ...