DHM - T1000 Holographic Transmission Microscope


Fast, robust and accurate characterization of transparent samples

The DHM T1000 is a transmission configuration microscope, suitable for transparent or semi-transparent samples. In this configuration, DHM not only provides information about the specimen surface, but is also capable of detecting structures located inside the material.

In transmission, DHM provides real-time measurements of optical path length with an accuracy of better than ?/600, providing a very high sensitivity to thickness or volume variations as well as to refractive index changes.

DHM T1000 microscopes are particularly well adapted for characterizing purely phase objects, such as biological cells, without contrast agents and at very low illumination power, thus providing unrivalled possibilities for non-invasive observations in life science applications.

Its application fields includes:

  Pharma and biotechnology   Food industry
  Cell biology   Genomics and proteomics
  High throughput screening   Diagnostics
  Life science   Material science
  Micro Optics   ...

Its single frame acquired hologram allows fast event monitoring (camera rate) and stroboscopic synchronisation. Moreover, the short acquisition time gives robust measurements. The DHM stability allows long time period investigations.

The set-up configuration allows standard optical microscope objective to be mounted on the DHM T1000. Select the objective that fulfils the field of view and lateral resolution you need.

The DHM& T1000's possibilities are revealed by the Lyncée Tec patented Koala Software that interprets the acquired hologram.   


DHM 1000 Family brochure (.pdf 798 kB) 

Real-time imaging

The acquisition and digital reconstruction rate (15 fps standard, faster in option) allows real-time imaging. Dynamic event viewing and active interaction with the observed phenomena are therefore possible.

Robust & stable

The short acquisition time (a few micro-seconds) makes the method almost insensitive to external vibrations and makes a vibration isolation table unnecessary. The robustness and stability of DHM™ permit stable measurements over long time periods to detect weak and slow deformations or movements.

High resolution

Interferometric axial resolution, less than 1 nanometer, along the vertical (Z) axis. Transverse resolution (in XY plane) defined by the numerical aperture of the objective (up to 300nm with oil immersion objectives), as with classical optical microscopy.

Non-contact & strictly non-invasive

Low-power visible light sample illumination (at least 10'000x less than confocal microscopy), no contact with the specimen surface, DHM™ ensures total preservation of your sample characteristics. Moreover, biological specimens can be observed without contrast agents, thus suppressing any type of chemical or physical disturbance.

Cost effective solution

DHM™ instruments have very low installation and operating costs. Adaptability and flexibility makes them very competitive in the high resolution microscopy domain. All these features make DHM™ instruments cost effective tools for R&D and quality control in production.


No sample preparation, no specific environments (temperature, vacuum, ...), no high precision positioning or orientation of the sample, the simplicity of DHM™ techniques makes it a user-friendly tool for quick and reliable measurements.

Powerful 3D software

Permits different operating modes with the same instrument to broaden the range of your applications. Offers unique DHM™-specific digital tools that improves the instrument's ease of use and robustness, as well as the measurement accuracy and stability. Implements standards and advanced measurements interfaces and external control possibilities in a convivial and flexible environement.