Illumination systems

CoolLED: pE4000 Universal LED Illumination System for Fluorescence Microscopy

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The CoolLED pE-4000 sets the standard as the universal illumination system for fluorescence microscopy.  The system has 16 selectable LED wavelengths that can be matched to the filters and fluorophores of almost any microscope, making it the broadest spectrum of illumination available. For the user who wants consistency and ease of use with simple on/off and intensity buttons, the pE-4000 can be operated as a ‘White’ illumination system. For the user who wants total control over the excitation source to improve imaging, individual wavelengths settings can be selected and controlled in “Advanced” mode.

As the first company to introduce LEDs into fluorescence microscopy, CoolLED has developed a comprehensive understanding of the complexities of configuring and selecting filter sets for samples which have multiple stains.  These ’multi-band’ filters must have a corresponding absorption and emission band for each stain and the bands must be kept spectrally separate to ensure there is no overlap.

CoolLED’s innovation comes from recognizing that all the stains used in multi-band work can have their absorption bands divided into four separate groups across the spectrum due to the restricted bandwidth available.  This has allowed the development of a patent-pending, wavelength-grouping concept which makes it possible to deliver more power in an efficient four channel system.

Wavelength grouping graphic without logo

With its unique 16 selectable LED wavelengths, the pE-4000 ensures optimum compatibility with all single and multi-band filter sets.  The flexibility and extensive functionality of the pE-4000 broadens the range of illumination options in core facilities.

pe 4000 01pE 4000 channel table

DAPI/FITC/TRITC/Cy5 Quad filter excited by pE-4000 at matched wavelengths shown above  

The compact pE-4000 Light Source houses all the LEDs, their combining optics, thermal management and control electronics.  A dual mode control pod gives manual control in either White or Advanced modes.  Light is delivered to the microscope by either a single liquid light guide (LLG) or fiber. LLGs can be used in conjunction with CoolLED’s pE-Universal Collimator which can accept a microscope adaptor from CoolLED’s extensive range.  

 Download datasheet here.

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