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Becker and Hickl: DCS-120 MACRO System Runs Fast FLIM

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Figures: Left: DCS-120 MACRO with FASTAC FLIM system. Right: FLIM image with 512 x 512 pixels, 1024 time channels, acqusition time   2 seconds. Image created by online-FLIM algorithm of SPCM data acquisition software.

The Becker and Hickl DCS-120 MACRO scanner can now be combined with Becker and Hickl's FASTAC fast-acqisition FLIM system. The system uses confocal scanning of an image area as large as 15 x 15 mm, and FLIM recording in four parallel SPC-150N TCSPC modules. Image formats are from 64 x 64 pixels up to 2048 x 2048 pixels, with up to 1024 time channels. Images of 256 x 256 pixels can be recorded within less than 0.5 seconds, images of 512 x 512 pixels within less than 2 seconds. Importantly, the system reaches high count rate and short acquition time without compromise in time resolution. FLIM data can still be recorded at sub-ps time channel with and sub-25 ps IRF width, thus fully exploiting the superiour time resolution of the bh TCSPC FLIM modules and ultra-fast hybrid detectors. It is therefore equally suitable for fast FLIM and precision FLIM applications. Moreover, multi-dimensional recording techniques like temporal mosaic FLIM, FLITS, and simultaneous FLIM/PLIM remain available.

For more information please see: 

1. Fast-Acquisition TCSPC FLIM System with sub-25 ps IRF Width. Application note. (click to download)

2. DCS-120 confocal and multiphoton FLIM systems, user handbook, 7th edition (2017) (click to download) 

 

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