Abberior Instruments: easy3D STED + RESCue STED = unrivaled 3D STED resolution
Actin stain using Abberior STAR RED Phalloidin. Samples were prepared by Dr. Christian Vogl, InnerEarLab, UMG Göttingen, Germany.
This xz-slice through mouse inner-ear hair cells using Abberior Instruments easy3D STED and RESCue STED demonstrates the unrivaled performance that can be achived by applying both techniques simultaneously. Since standard 3D superresolution places more photoinduced stress on the marker, the key ingredients that made this image possible where 1st: producing a STED-zero point of exceptional quality using easy3D STED, and 2nd: further reducing the light dose in the sample with RESCue STED.
Three dimensional live-cell STED microscopy at increased depth using a water immersion objective
Spherical abberations corrected z-STED raw data
Our patent protected easy3D STED technology produces superior 3D STED data – please check out our new publication:
J. Heine, C. A. Wurm, J. Keller-Findeisen, A. Schönle, B. Harke, M. Reuss, F. R. Winter, G. Donnert, "Three dimensional live-cell STED microscopy at increased depth using a water immersion objective", Review of Scientific Instruments (2018)
Abberior Instruments: easy3D STED + RESCue STED = unrivaled 3D STED resolution