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Becker and Hickl DCS-120 FLIM system records X-Y mosaics

bpae mosaic 16 11 04 2000 2800 txtbig comp160

bpae mosaic 16 11 04 2000 2800 detail2 comp160

Above: FLIM mosaic of BPAE cell scample, 248x248 pixels, 256 time channels. Below: Zoom into data shown above.

With software version 9.76, the control of a motorised sample stage has been integrated in the Becker and Hickl SPCM TCSPC/FLIM data acquisition software. In combination with the Mosaic FLIM function of SPCM, the sample stage can be used to record arrays of FLIM images with the Becker and Hickl DCS-120 confocal and multiphoton FLIM systems. The FLIM system scans an image at one position of the sample, then offsets the sample by the size of the scan area, and scans a new image. The process is repeated, combining the images of the individual frames into a single, large x-y-t data set. Images covering an area of several mm diameter can be obtained with high-NA objective lenses. Compared to a single scan with a low-magnification objective lens, a higher collection, a higher 2p excitation efficiency, and a higher optical resolution is obtained.

For details please see application note. Please click here to download

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