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Abberior STEDYCON: Imaging beyond barriers

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Abberior Instruments is known for extraordinary customization ability of its flexible STED microscope platform. We now take things in a different direction. We have developed a completely new instrument, the ultimate compact STED plus confocal microscope, called STEDYCON.

STEDYCON is a completely new class of nanoscope. It converts your conventional epifluorescence microscope into a powerful multicolor confocal and STED system! At the same time, it is incredibly compact and can be used by anyone!

  • Convert your existing microscope - Instantly upgrade any epifluorescence microscope body to multicolor confocal and STED capabilities.
  • Get top-notch STED performance - With STEDYCON your microscope will image at 30nm resolution.
  • Save Space - The incredibly compact STEDYCON comes at the size of a camera.
  • Plug & Play - The STEDYCON is assembled within minutes. It simply connects to the C-mount camera port of your microscope with no need for optical alignment.
  • Service and maintenance free - The laser beams of the STEDYCON are aligned by design as provided by its patent-protected 'easySTED' optical arrangement.
  • Innovative web-based user interface - The STEDYCON can be controlled via a browser on any PC, MAC or even a tablet!

Product Views

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Browser Based Cross Platform Software

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The STEDYCON can be controlled via Abberior Instruments STEDYCON smart control, a web-browser-based graphical user interface. STEDYCON smart control was designed from scratch to provide the highest possible usability completing the overall STEDYCON concept of easy installation, intuitive usage, and maintenance-free operation. Beginners can image with super-resolution within minutes. Smart control runs on every device with a modern web browser like Windows-based PCs, Linux-based PCs, Apple PCs or even tablets.

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Gattaquant STED-AFM ruler (70 nm distance between emitters) were imaged using a STEDYCON attached to a Zeiss Axio Imager Z2 and a Nikon Eclipse Ni. Shown is RAW DATA.

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Tubulin cytoskeleton of mammalian cells labelled with Abberior STAR635P. Shown is RAW DATA. Images were acquired using a STEDYCON attached to a Zeiss Axio Imager Z2.

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Nuclear pore complex subunits in the central channel (nup153) of mammalian cells immunolabelled using Abberior STAR635P. Shown is RAW DATA. Images were acquired using a STEDYCON attached to a Zeiss Axio Imager Z2.

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Two subunits of the nuclear pore complex were immunolabelled using antibodies against gp210 and antibodies with multiple specificities (PAN4/5) and secondary antibodies coupled to Abberior STAR580 and Abberior STAR635P. Note that gp210 is localized in an eightfold symmetric structure at the rim of the nuclear pore complex. Shown is RAW DATA. Images were acquired using a STEDYCON attached to a Zeiss Axio Imager Z2.

Abberior specifications:

 

  • Laser lines
  • Excitation laser lines: 450nm, 594nm, 640nm (all pulsed)
  • STED laser: 775nm (pulsed)
  • 2D STED resolution: <40nm, typically 30nm (depending on dyes and objectives used)
  • Easy-STED geometry: all laser lines aligned by design; maintenance free optical beam path
  • 3 confocal imaging channels
  • Detection: APD1: 650-700nm; APD2: dual-band detection: 502-572nm & 608-627nm, gated detection (for confocal and STED)
  • QUAD beam scanner: 90µm x 80µm (with a 100x objective); 512x512 pixel up to 1.1 frames/s; line frequency up to 800Hz
  • Pinhole: 1.1 Airy unit for a 100x objective
  • Software: browser-based control software operational on any modern PC, Mac or tablet: line interleaved STED and confocal imaging in three imaging channels; options to define z-stacks, acquisition queues and time series, auto-save function
  • Dimensions: STED-camera head: 11cm x 20cm x 20cm; controller: 22cm x 55cm x 60cm; total weight head plus controller unit < 40kg
  • STEDYCON is tested with different microscope stands and objectives from different vendors; contact us for details
  • Optionally: Optical table or antivibrational table, z-focusing piezo-drive, microscope stand and objective validated for STEDYCON

Download product brochure here and dimensional drawings here.

The Abberior STEDYCON is compatible with any modern upright or inverted microscope. The following bodies have been experimentally tested:

  • Zeiss Axio Imager Z2 (Upright) with objective 100x NA=1.46
  • Nikon Eclipse Ni (Upright) with objective 100x N=1.45 Lambda Series
  • Olympus IX83 (Inverted)

Please contact us to clarify the situation for your existing microscope.

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