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Becker & Hickl: DCS-120 Confocal FLIM Scanning Systems for Laser Scanning Microscopes

 DCS120

The Becker & Hickl DCS-120 system uses excitation by ps diode lasers or femtosecond titanium-sapphire lasers, fast scanning by galvanometer mirrors, confocal detection, and FLIM by bh’s multidimensional TCSPC technique to record fluorescence lifetime images at high temporal resolution, high spatial resolution, and high sensitivity. The DCS-120 system is available with inverted microscopes of Nikon, Zeiss, and Olympus. It can also be used to convert an existing conventional microscope into a fully functional confocal or multiphoton laser scanning microscope with TCSPC detection.

Due to its fast beam scanning and its high sensitivity the DCS-120 system is compatible with livecell imaging. DCS-120 functions include simultaneous recording of FLIM or steady-state fluorescence images simultaneously in two fully parallel wavelength channels, laser wavelength multiplexing, time-series FLIM, timeseries recording, Z stack FLIM, phosphorescence lifetime imaging (PLIM), fluorescence lifetime-transient scanning (FLITS) and FCS recording. Applications focus on lifetime variations by interactions of fluorophores with their molecular environment. Typical applications are ion concentration measurement, FRET experiments, autofluorescence imaging, and plant physiology.

triple exponential decay

  • Complete laser scanning FLIM microscopes
  • FLIM upgrade for conventional microscopes
  • PLIM - Phosphorescence lifetime imaging simultaneously with FLIM
  • One or two bh BDL-SMC picosecond diode lasers
  • Laser wavelengths 405 nm, 440 nm, 473, or 640 nm
  • Scanning by fast galvano mirrors
  • Frame times down to <100 ms, line times down to 1 ms
  • Frame scan, line scan, and point (FCS) capability
  • Two fully confocal detection channels
  • Individually selectable pinholes
  • Individually selectable filters
  • Secondary beamsplitter wheel with dichroic and polarising beamsplitters
  • High efficiency due to direct-coupled detectors
  • GaAsP Hybrid Detectors (HPM-100), PMC-100, H7422, ultra-fast MCP PMT, or SPAD detector modules
  • Multi-spectral FLIM detector module
  • Fully integrated scanning, detector and laser control
  • Single-channel and dual-channel (parallel detection) TCSPC systems
  • On-board image acquisition and time-tag imaging modes
  • Electronics integrated in bh Simple-Tau 150 or 152 or standard-PC systems
  • Instrument and data analysis software for Windows 2000, NT, XP, or Vista

Download further information here:

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