The new MicroTime 200 STED reaches super-resolution below 50 nm
PicoQuant has released a STED (stimulated emission depletion) add-on for the time-resolved confocal microscope MicroTime 200. This new super-resolution extension allows the MicroTime 200 to perform measurements that break the diffraction limit of light reaching lateral resolutions below 50 nm.
The STED add-on for the MicroTime 200 is based on the easy-STED principle. Excitation and STED laser are first coupled through a common fiber to ensure perfect alignment and shape of the beams. An optical element then changes the STED laser focus to the required donut-shape, while leaving the excitation laser unaffected. This simple implementation makes spatial alignment of the two laser beams unnecessary. Gated STED in which the excitation laser and detection is pulsed and synchronised and the STED depletion is continuous is also supported by the MicroTime 200 STED to enhance resolution in images even more. Test users of the new STED add-on appreciate the easy concept in a microscope that is still accessible by the researcher and open for modification. “This is simple super-resolution microscopy: We attached a STED-dye with click-chemistry and got impressive high resolution images of the E. coli chromosome,” says Mike Heilemann, professor at the Goethe-University Frankfurt.